Cell culture is an important technique in both cellular and molecular biology given that it provides the best platform for studying the normal physiology and biochemistry of cells. A cell is the basic structural, functional and biological unit of all living things.
In order to understand an organism or given tissues, it is important to understand how its cells work. This makes cell culture significantly important for vaccine development, screening (drugs etc.) and diagnosis of given diseases/conditions.
This course will also provide you with the basic information of Quality Control Considerations in Cell Culture, as The quality of materials used (cell lines, media and other reagents) will affect the quality of the cultures and the subsequent scientific data and products derived from them.
Identification of the component of cell culture unit and How the equipment’s (Safety cabinet, Inverted Microscope and CO2 incubator)
Acquiring & selecting (Adherent vs. suspension culture)
Culture environment: (media, pH, CO2, and Temperature)
Basic Constituents of Media
Different types of culture medium and their uses
preparation of media.
Sub culturing cells
Preparation of Media and subculture of adherent cells
Cryopreservation and Storage of Cells
Thawing frozen cells Counting cells in a hemocytometer
Trypan blue exclusion test of cell viability
MTT Assay and Data Analysis