Flow Cytometric Analysis

Flow cytometry is a standard laser-based technology that is used in the detection and measurement of physical and chemical characteristics of cells or particles in a heterogeneous fluid mixture. The use of flow cytometry has increased over the years as it provides a rapid analysis of multiple characteristics (both qualitative and quantitative) of the cells. Because of its speed and ability to scrutinize at the single-cell level, flow cytometry offers the cell biologist the statistical power to rapidly analyze and characterize millions of cells, albeit at the expense of the morphological characteristics and subcellular localization that microscopy can provide.

Data Analysis

Each cell that passes through the laser light is detected as a separate event. Also, different types of detected light: forward-scatter, side-scatter, and specific wavelengths of fluorescence emission, is assigned a distinct channel. The data for each of these events is plotted independently and can be represented by two methods: histograms and dot-plots.

Flow Cytometry Service Details

Requirements: Sample submission

Cells and antigens deteriorate with age, therefore the most reliable results are obtained on fresh samples
Frozen/ over-heated samples will be rejected.
Before application of the antibodies for flow cytometry, we usually check the sample for sufficient preservation and number of cells in question. If there are insufficient cells or the cells are mostly lysed, we will not proceed with the full flow cytometric evaluation.

Service Report

Once the lab work and data analysis are complete, we send you a service report including:

List of antigens and type of immunofluorescence analysis carried out.
Quality of the sample, in terms of viability.
Data analysis ( plots ) .
Morphologic description (when applicable).
An interpretive conclusion
Complete methodology part .

Time Line

Turnaround time is about 1-3 weeks. Actual times vary based on how many projects we have.

Types

Cell health status—from viability to late-stage apoptosis or programmed cell death.
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Cell cycle status—providing a powerful tool to assess cells in G0/G1 phase versus S phase, G2, or polyploidy, including analysis of cell proliferation and activation
Understanding the immune cell subset phenotype and activation/functional status (cellular immunome) prior to and post therapy “biomarkers “.

Quotation Request Form

Getting started is easy. Complete this form with the information that we need in order to set up your screening service.