
Winter Trainings
Genetic testing of embryos and Infertility

The Evolving Role of Genetic Tests in Reproductive Medicine
1 Month | 30 Hours | 1000 EGP
Semen analysis (physical, chemical & microscopic examination).
Sperm DNA Fragmentation (SDF) & Sperm Chromatin Desperation (SCD).
Chromosomal abnormalities (numerical & structural).
Karyotype training cut-and-paste by newer automatic software method and idiograming.
Preimplantation Genetic Testing (PGT).
AZF deletion testing.
Basics of Molecular Biology

Essential Molecular Biology Lab Skills
4 Day | 4 Hours | 400 EGP
Introduction to molecular biology techniques.
Principles of nucleic acid extraction.
Practices & applications of PCR.
Agarose Gel Electrophoresis.
Cytogenetics

Human & Animal Chromosomal Analysis
2 Weeks | 25 Hours | 1000 EGP
Set up of blood culture, cell harvesting and slides preparation.
Metaphase chromosome preparations and G-Banding.
Microscope training, examination and metaphase drawing method.
Karyotype training cut-and-paste by newer automatic software method and idiograming.
Identification of numerical and structural abnormalities of Chromosomes.
International system for cytogenetic nomenclature (ISCN).
Fluorescent in Situ Hybridization (FISH).
Diagnosis of chromosomal aneuploidy and micro-deletions.
Western Blotting

Hands-On Workshop
3 Days | 15 Hours | 1500 EGP
Protein extraction techniques
SDS-PAGE, Separation of proteins through a gel.
Antibodies Selection.
Transfer from gel to membrane.
Primary and Secondary antibody staining.
Protein detection and Visualization.
Appling the appropriate controls for western blot analysis.
Troubleshooting and the most common problems in western blot
Cell Culture

Principles & Practice of Cell Culture
3 Weeks | 25 Hours | 1000 EGP
Maintenance and handling cell lines
Measurement of cellular viability and cytotoxicity assays
Identification of bacterial & fungal contamination
Cryopreservation - freezing cells
Anti-proliferative effects of natural products against various cancer cell lines
Molecular Biology Program

Medical & Agricultural Applications
2 Weeks | 25 Hours | 800 EGP
Principles of nucleic acid extraction from different sources
Practices and applications of polymerase chain reaction (PCR) and Real-Time PCR.
Mutation Detection using molecular techniques.
Assessment of gene expression levels.
Gel electrophoresis images analysis & construction of phylogenetic tree
Conservation of Genetic Diversity & its application in Gene Banks.
Unloading the secretes of the genome using next-generation sequencing –NGS.