Summer Training 2024

• Principles of nucleic acid extraction from different sources.

• Practices and applications of polymerase chain reaction (PCR) and Real-Time PCR.  

• Agarose Gel Electrophoresis (Preparation tricks - Troubleshooting - How to Interpret results) 

• Reverse transcription-polymerase chain reaction: the technique and its application.  

• Real-time PCR (Execution of the experiments - Fluorescent Chemistries - Quantification methods - Data Analysis using qPCR Software - Validation criteria- How to deal with the machine)  

• Quantitative Detection and Viral Load Analysis 

• Detection of selected antibiotic resistance genes by using Multiplex PCR assay on Clinical Outcomes  

• Application of Nested Polymerase Chain Reaction Assay to Detect infectious agents from Nasal Swabs  

• Single nucleotide polymorphism genotyping using allele-specific PCR and fluorescence melting curves  

• Assessment of gene expression levels in plants under different types of stress  

• Clinical, molecular, and genotype–phenotype correlation studies  

• Use of genomic information to optimize therapy.  

• Diagnostic genetic tests that could help predict response to therapy 

• Quality control in diagnostic tests.

• Principles and applications of modern DNA sequencing.  

• Unloading the secretes of the genome using next-generation sequencing – NGS

Bioinformatics Part

• Primer Design  

• Sequence Alignment  

• Variations & Mutagenesis  

• Snap Gene 

• Understanding cancer epidemiology, risk factors, symptoms, types & stages.  

• The role of molecular biology in cancer diagnosis & treatment.  

• Studying the genes whose mutation contributes to the causation of cancer.  

• Ras genes mutation triggering the development of different types of human tumors.  

• P53 mutations & programmed cell death.  

• Prognostic Implication of FLT3 Gene Mutations in Acute Myeloid Leukemia  

• In vitro anti-cancer effect of different treatments in Breast cancer cell line  

• Diagnostic role of BCR-ABL in Acute Lymphatic Leukemia  

• In Vivo Cancer Models to Guide Precision Medicine & study Efficacy of Cancer Therapy  

• BRCA1 and BRCA2 genes mutations & breast cancer development  

• Identifying gene expression patterns associated with drug response  

• Calculating minimal residual disease (MRD)  

• Understanding major molecular response (MMR)  

• Identifying prognosis relevant genes  

• Current approaches and challenges in managing and monitoring treatment response in cancer patients  

• Targeted mutation detection in clinical cancer settings: formaldehyde fixed paraffin embedded (FFPE) tumor tissues 

• Principles of Nucleic Acid Extraction.

• DNA Extraction using silica-based method & troubleshooting.

• Polymerase Chain Reaction - PCR.

• PCR reaction set-up, thermal profile , troubleshooting.

• Agarose Gel Electrophoresis.

• Introduction to Forensic Science and Criminalistics 

• Forensic body fluid identification  

• STR & VNTR analysis for personal identification  

• Molecular DNA Analysis in Forensic Identification

• Microscope training, examination and metaphase drawing method 

• Karyotype training cut-and-paste by newer automatic software method and idiograming 

• Reading the chromosomes banding 

• Identification of numerical and structural abnormalities of Chromosomes. 

• International system for cytogenetic nomenclature (ISCN) 

• Diagnosis of chromosomal aneuploidy and micro-deletions 

• Cytogenetics characterization & evaluation

• QC Microbiology lab Staff and Design 

• QC Microbiology Lab Equipment 

• QC Microbiology Lab Quality System 

• QC Microbiology Lab Tests and Activities 

• Aseptic Technique, Staining, Microbial culturing Techniques & Growth Promotion Technique   

• Environmental Monitoring 

• Instructions: Safety and contamination control 

• Handling of Petri-dishes, inoculation and purification  

• Staining of Bacterial and Microscopic Examination  

• Practical Culturing Techniques  

• Environmental Swabbing Technique 

• ISO Management Systems 

• Available Job opportunities in QC 

• Working Environment 

• Qualifications and Training 

• Career Path  

Chemistry analysis 

• Measuring blood glucose level 

• Measuring total cholesterol level & how to detect good & bad cholesterol 

• Liver function test 

• Kidney function test 

Serology 

• Examine elements of the human immune system and its response to foreign bodies & detection of infections using serological techniques. 

• Measuring the level of C - reactive protein (CRP) 

• Antistreptolysin O Titer (ASO) Test 

• Rheumatoid Factor (RF) Test 

Molecular biology analysis 

• Applications of molecular biology techniques in the diagnosis and monitoring of infectious diseases at the nucleic acid level. 

• Using PCR-based systems to detect the etiologic agents of disease directly from clinical samples, without the need for culture. 

Hematology 

• Blood sampling 

• Blood group test 

• ESR blood test & clinical applications  

• Examining blood films slides under the microscope 

• Analyzing abnormalities of quantity & quality of blood cells 

• How to read a laboratory report correctly

Microbiology

• Microbiology lab safety and QC. 

• Microbial culture media preparation 

• Bacteriological culture methods and techniques 

• Gram stain principle, procedure interpretation 

• Antibiotic sensitivity testing 

Parasitology 

• Diagnosis of human parasitic infections 

• Detection of Parasites in urine and stool samples  

• Semen analysis (physical, chemical & microscopic examination) 

• Sperm DNA Fragmentation (SDF) & Sperm Chromatin Desperation 

• (SCD) 

• In vitro fertilization (reasons & process) 

• Pedigree definition & interpretation 

• Chromosomal abnormalities (numerical & structural) 

• Preimplantation Genetic Testing (PGT)