Mid Year
Trainings 2024

• Extraction of active ingredients from different plant species using individual  and succession extraction methods

• Determination of primary and secondary metabolites in each sample.

• Separation and identification of active ingredients using column  chromatography and spectroscopic methods.

• In vitro assays to investigate the anti-inflammatory activity of herbal extracts.

• Biosafety recommendations on the handling of animal cell cultures.

• MTT assay to evaluate the cytotoxic potential of a drug.

• Cryopreservation of cells for clinical application .

• Principles of Nucleic Acid Extraction.

• DNA Extraction using silica-based method & troubleshooting.

• Polymerase Chain Reaction - PCR.

• PCR reaction set-up, thermal profile , troubleshooting.

• Agarose Gel Electrophoresis.

• Molecular Markers and Genome Editing. 

• SCoT and ISSR for detecting genetic polymorphism.

• Hematology analysis

• Serology 

• Immunology

• Parasitology

• Chemistry analysis

• Microbiology analysis

• Molecular biology analysis

Molecular Genetics:  

• The training program delves into gene expression analysis, which is a  fundamental aspect of molecular genetics. Participants will learn about the  NHX gene and its expression under salinity stress in bread wheat.

 • Understanding gene expression patterns and molecular mechanisms can  provide insights into plant responses to environmental factors, including  salinity. This knowledge can further contribute to the development of molecular  breeding strategies for improving crop traits.  

• The practical sessions, such as qPCR, RT-PCR, and multiplex PCR, are key  molecular genetics techniques used for gene expression analysis, genetic  profiling, and detection of genetic variations and mutations. 

 Economics:  

• The training program's focus on molecular diagnostics and quantification  techniques has significant implications in economics, particularly in the fields  of biotechnology and healthcare. 

• Molecular diagnostics plays a crucial role in disease diagnosis and monitoring,  which can have economic implications in terms of healthcare costs, treatment  strategies, and disease control measures. 

• The ability to accurately quantify gene expression levels using techniques like  qPCR and RT-PCR can aid in evaluating the effectiveness of agricultural  interventions, such as breeding programs or genetic engineering approaches,  potentially impacting crop yields and food security. 

• The workshop's coverage of antimicrobial resistance and its detection can also  have economic implications by informing appropriate antibiotic usage,  reducing treatment failures, and minimizing healthcare costs associated with  multidrug-resistant infections.